Treatment with atrial natriuretic peptide induces adipose tissue browning and exerts thermogenic actions in vivo

Abstract Increasing evidence suggests natriuretic peptides (NPs) coordinate inter-organ metabolic crosstalk with adipose tissues and play a critical role in energy metabolism. We recently reported A-type NP (ANP) raises intracellular temperature cultured adipocytes low-temperature-sensitive manner. herein investigated whether exogenous ANP-treatment exerts significant impact on vivo. Mice fed high-fat-diet (HFD) or normal-fat-diet (NFD) for 13 weeks were treated without ANP infusion subcutaneously another 3 weeks. significantly ameliorated HFD-induced insulin resistance. HFD increased brown tissue (BAT) cell size the accumulation of lipid droplets (whitening), which was suppressed by (re-browning). Furthermore, induced enlarged inguinal white (iWAT), crown-like structures epididymal WAT, hepatic steatosis, all substantially attenuated ANP-treatment. Likewise, markedly UCP1 expression, specific marker BAT, iWAT (browning). also further expression BAT NFD. Accordingly, cold tolerance test demonstrated ANP-treated mice tolerant to exposure. In summary, administration ameliorates resistance attenuating steatosis inducing browning (activation thermogenic program), leading vivo thermogenesis during